Description |
Goat anti-RANBPM / RANBP9 Antibody Principle Name: RANBPM / RANBP9 Official Symbol: RANBP9 All Name and Symbols: RANBP9; RanBPM; RAN binding protein 9; RANBPM; novel centrosomal protein RanBPM; ran binding protein, centrosomal Accession Number(s): NP_005484.2 Peptide Sequence: GSCAFATVEDYLH, is from C Terminus Human Gene ID(s): 10048 Non-Human GeneID(s): 56705 (mouse); Important Comments: This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC, ICC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1ug/ml. Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration: 3ug/ml. Reference(s): Wang D, Li Z, Messing EM, Wu G. Activation of Ras/Erk pathway by a novel MET-interacting protein RanBPM. J Biol Chem. 2002 Sep 27; 277(39):36216-22. Epub 2002 Jul 29..PMID: 12147692 |