Goat anti-RANBPM / RANBP9 Antibody

Goat anti-RANBPM / RANBP9 Antibody
Principle Name: RANBPM / RANBP9
Official Symbol: RANBP9
All Name and Symbols: RANBP9; RanBPM; RAN binding protein 9; RANBPM; novel centrosomal protein RanBPM; ran binding protein, centrosomal
Accession Number(s): NP_005484.2
Peptide Sequence: GSCAFATVEDYLH, is from C Terminus
Human Gene ID(s): 10048
Non-Human GeneID(s): 56705 (mouse);
Important Comments: This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC, ICC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1ug/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration: 3ug/ml.
Reference(s): Wang D, Li Z, Messing EM, Wu G. Activation of Ras/Erk pathway by a novel MET-interacting protein RanBPM. J Biol Chem. 2002 Sep 27; 277(39):36216-22. Epub 2002 Jul 29..PMID: 12147692