Description |
Goat anti-FBXL12 Antibody Principle Name: FBXL12 Official Symbol: FBXL12 All Name and Symbols: FBXL12; likely ortholog of mouse f-box and leucine-rich repeat protein 12; FLJ20188; hypothetical protein FLJ20188; F-box and leucine-rich repeat protein 12; Fbl12 Accession Number(s): NP_060173.1 Peptide Sequence: RACPKESMDWWM, is from C Terminus Human Gene ID(s): 54850 Non-Human GeneID(s): 30843 (mouse); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Preliminary experiments gave no signal but low background in Human Brain lysates at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Lung shows staining of ciliated epithelial cells in the bronchioles. Recommended concentration, 4-6ug/ml. Reference(s): Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins. Curr Biol. 1999 Oct 21; 9(20):1180-2..PMID: 10531037 |