Goat anti-DKK3 / REIC Antibody

Goat anti-DKK3 / REIC Antibody
Principle Name: DKK3 / REIC
Official Symbol: DKK3
All Name and Symbols: DKK3; REIC; dickkopf homolog 3 (Xenopus laevis); DKK-3; RIG-like 5-6; RIG-like 7-1; dickkopf 3; Dickkopf gene 3; dickkopf (Xenopus laevis) homolog 3; dickkopf homolog 3; RIG; regulated in glioma
Accession Number(s): NP_037385.2; NP_056965.3; NP_001018067.1
Peptide Sequence: AAAALLGGEEI, is from C Terminus
Human Gene ID(s): 27122
Non-Human GeneID(s):
Important Comments: NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx. 70kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene
Immunohistochemistry: In paraffin embedded Human Brain Cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration: 5-10ug/ml.
Reference(s): Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Functional and structural diversity of the human Dickkopf gene family. Gene. 1999 Oct 1; 238(2):301-13..PMID: 10570958