Description |
Goat anti-Dysadherin Antibody Principle Name: Dysadherin Official Symbol: FXYD5 All Name and Symbols: FXYD5; dysadherin; FXYD domain containing ion transport regulator 5; RIC; IWU1; IWU-1; dysad; HSPC113; KCT1; OIT2; PRO6241; FXYD domain-containing ion transport regulator 5; keratinocytes associated transmembrane protein 1; DYSAD; HSPC113 Accession Number(s): NP_054883.3; NP_659003.1; NP_001158077.1 Peptide Sequence: GKCRQLSRLCRNHCR, is from C Terminus Human Gene ID(s): 53827 Non-Human GeneID(s): Important Comments: NP_054883.3, NP_659003.1and NP_001158077.1 are variants that represent the same protein. Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 8000. Western Blot: Approx 38kDa band observed in Human Spleen lysates (calculated MW of 19.5 kDa according to NP_054883 and NP_659003). The band was successfully blocked by incubation with the immunizing peptide. According to the literature this protein is O- Reference(s): Ino Y, Gotoh M, Sakamoto M, Tsukagoshi K, Hirohashi S. Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. Proc Natl Acad Sci U S A. 2002 Jan 8; 99(1):365-70..PMID: 11756660 |