SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity - 1 kit

ELISA + FRET; Protease activity

Matrix Metalloproteinase (MMP); MMP-2

Dry Ice

The SensoLyte Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. A monoclonal anti-human-MMP-2 antibody is employed to pull down MMP-2 from the mixture first. MMP-2 activity is then quantified by a 5-FAM/QXL 520 fluorescence resonance energy transfer (FRET) peptide. The fluorescence signal is monitored at Ex/Em=490 nm/520 nm upon MMP-2-induced cleavage of the FRET substrate. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. Ample materials are provided to perform 96 assays in a 96-well format.

Adhesion & Extracellular Matrix; Cancer & Apoptosis / 0

520

Fluorescent

Research use