VCAM1, 25-698 aa, Human, His tag, E.coli

VCAM1 is a member of the Ig superfamily and is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Recombinant human VCAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.25 mg/ml (determined by Bradford assay)

Wu TC (2007) Cancer Res. 67 (13): 6003-6.

>, 80% by SDS - PAGE

MGSSHHHHHH SSGLVPRGSH MFKIETTPES RYLAQIGDSV SLTCSTTGCE SPFFSWRTQI DSPLNGKVTN EGTTSTLTMN PVSFGNEHSY LCTATCESRK LEKGIQVEIY SFPKDPEIHL SGPLEAGKPI TVKCSVADVY PFDRLEIDLL KGDHLMKSQE FLEDADRKSL ETKSLEVTFT PVIEDIGKVL VCRAKLHIDE MDSVPTVRQA VKELQVYISP KNTVISVNPS TKLQEGGSVT MTCSSEGLPA PEIFWSKKLD NGNLQHLSGN ATLTLIAMRM EDSGIYVCEG VNLIGKNRKE VELIVQEKPF TVEISPGPRI AAQIGDSVML TCSVMGCESP SFSWRTQIDS PLSGKVRSEG TNSTLTLSPV SFENEHSYLC TVTCGHKKLE KGIQVELYSF PRDPEIEMSG GLVNGSSVTV SCKVPSVYPL DRLEIELLKG ETILENIEFL EDTDMKSLEN KSLEMTFIPT IEDTGKALVC QAKLHIDDME FEPKQRQSTQ TLYVNVAPRD TTVLVSPSSI LEEGSSVNMT CLSQGFPAPK ILWSRQLPNG ELQPLSENAT LTLISTKMED SGVYLCEGIN QAGRSRKEVE LIIQVTPKDI KLTAFPSESV KEGDTVIISC TCGNVPETWI ILKKKAETGD TVLKSIDGAY TIRKAQLKDA GVYECESKNK VGSQLRSLTL DVQGRENNKD YFSPE