ArtNr |
ARP-01-P1473-10 |
Hersteller |
American Research Products
|
Menge |
10 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
ECLASS 5.1 |
34160400 |
ECLASS 6.1 |
34160400 |
ECLASS 8.0 |
42020190 |
ECLASS 9.0 |
42020190 |
ECLASS 10.0.1 |
32160409 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Description |
OTC, also called ornithine carbamoyltransferase, belongs to the ATCase/OTCase family. OTC plays a vital role in the urea cycle, catalyzing the second step in this pathway: the formation of L-citrulline from L-orthinine and carbamoyl phosphate. In humans, the urea cycle is an important pathway to detoxification of ammonia. Mutations in the gene encoding OTC are associated with the X-linked disorder OTCD (ornithine carbamoyltransferase deficiency). OTCD is a disorder of the urea cycle characterized by hyperammonemia. Recombinant human OTC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form |
Liquid. In 20mM MES buffer (pH 6.0) containing 2mM DTT, 10% glycerol, 100mM NaCl |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
ornithine carbamoyltransferase, OCTD, OTCase |
Concentration |
0.5mg/ml (determined by Bradford assay) |
NCBI |
NP_000522 |
References |
- Trivedi, M., et al. (2001) J. Clin. Gastroenterol. 32: 340-343.
- Yamaguchi, S., et al. (2006) Hum. Mutat. 27: 626-632.
|
Antigen Recognized in Species |
Human |
Purity |
>, 90% by SDS - PAGE |
Molecular Weight |
38.9kDa (347aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMNKVQL KGRDLLTLKN FTGEEIKYML WLSADLKFRI KQKGEYLPLL QGKSLGMIFE KRSTRTRLST ETGFALLGGH PCFLTTQDIH LGVNESLTDT ARVLSSMADA VLARVYKQSD LDTLAKEASI PIINGLSDLY HPIQILADYL TLQEHYSSLK GLTLSWIGDG NNILHSIMMS AAKFGMHLQA ATPKGYEPDA SVTKLAEQYA KENGTKLLLT NDPLEAAHGG NVLITDTWIS MGQEEEKKKR LQAFQGYQVT MKTAKVAASD WTFLHCLPRK PEEVDDEVFY SPRSLVFPEA ENRKWTIMAV MVSLLTDYSP QLQKPKF |
Gene Category |
Metabolism |
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