ArtNr |
ARP-01-P1237-20 |
Hersteller |
American Research Products
|
Menge |
20 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Description |
DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in both breast and ovarian tissues. DUSP3 Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. Recombinant human DUSP3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form |
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl. |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Dual specificity protein phosphatase 3, VHR |
Concentration |
1 mg/ml (determined by Bradford) |
NCBI |
NP_004081 |
References |
- Todd JL., et al. (1999) J Biol Chem. 274(19):13271-80.
- Keyse SM., et al. (1995) Biochim Biophys Acta. 1265(2-3):152-60.
|
Antigen Recognized in Species |
Human |
Purity |
>, 95 % by SDS - PAGE |
Molecular Weight |
22.6 kDa (205aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP |
Gene Category |
Cancer |
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