ArtNr |
ARP-01-P0912-20 |
Hersteller |
American Research Products
|
Menge |
20 ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Description |
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Form |
Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl. |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
Dihydrofolate reductase, EC 1.5.1.3 |
Concentration |
1 mg/ml (determined by Bradford assay) |
NCBI |
NP_034179 |
References |
- Chen MJ., et al. (1984) J Biol Chem. 259(6):3933-43.
- Cody V., et al. (2009) Biochemistry. 48(8):1702-11.
|
Antigen Recognized in Species |
mouse |
Purity |
>, 95% by SDS - PAGE |
Molecular Weight |
23.8 kDa (207aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD |
Gene Category |
Redox Proteins |
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