Hersteller |
American Research Products
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Menge |
0.5 mg |
ArtNr |
ARP-01-1812-0.5 |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Description |
Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active. Recombinant human BPGM, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques |
Form |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
Storage |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Synonyms |
BPG-dependent PGAM, Bisphosphoglycerate mutase 2, 3-bisphosphoglycerate mutase, erythrocyte, 2, 3-bisphosphoglycerate mutase, 2, 3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192. |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
NCBI |
NP_001715 |
References |
- Pritlove DC., et al. (2006) Placenta. 27(8):924-7
- Wang Y., et al. (2006) J. Biol. Chem. 281(51):39642-8.
|
Antigen Recognized in Species |
Human |
Purity |
>, 95% by SDS - PAGE |
Molecular Weight |
31kDa (267aa), confirmed by MALDI-TOF |
Amino Acid Sequence |
MSKYKLIMLR HGEGAWNKEN RFCSWVDQKL NSEGMEEARN CGKQLKALNF EFDLVFTSVL NRSIHTAWLI LEELGQEWVP VESSWRLNER HYGALIGLNR EQMALNHGEE QVRLWRRSYN VTPPPIEESH PYYQEIYNDR RYKVCDVPLD QLPRSESLKD VLERLLPYWN ERIAPEVLRG KTILISAHGN SSRALLKHLE GISDEDIINI TLPTGVPILL ELDENLRAVG PHQFLGDQEA IQAAIKKVED QGKVKQAKKL EHHHHHH |
Gene Category |
Enzymes |
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