Anti-ORAI1

ORAI1(ORAI calcium release-activated calcium modulator 1), also known as A CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selectiveA ion channelA that in humans is encoded by theA ORAI1A gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causesA severe combined immunodeficiency(SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4A transmembraneA domains and form tetramers.A Prakriya et al.(2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.A Prakriya et al. (2006)A showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

Lyophilized

2-8C. For long term -20C after reconstitution.

1. Prakriya, M., Feske, S., Gwack, Y., Srikanth, S., Rao, A., Hogan, P. G.A Orai1 is an essential pore subunit of the CRAC channel.A Nature 443: 230-233, 2006.

Immunogen affinity purified.