ArtNr |
ARP-03-16043 |
Hersteller |
American Research Products
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Background |
Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues. |
Immunogen |
Histamine conjugated to BSA |
Form |
Serum containing 0.1% sodium azide |
Specificity |
Absorption with 10-100 ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagons and histidine do not. |
Working Dilution |
1:50-1:100 (PAP), 1:15 - 1:30 (immunofluorescence microscopy) |
Storage |
2-8C for immediate use, or at -20C (aliquot) |
References |
- Hakansson R et al. (1986) Histochemistry 86: 5-17
- Johansson O et al. (1992) Histochem J 24: 283-287
- Axelson J et al. (1990) Gastroenterology 99: 635-640
|
Antigen Recognized in Species |
Human, Rat, Mouse, and most mammals |
Positive Control |
Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin sections (vapor fixed in diethylpyrocarbonate, DEPC) of rat stomach (see ref.) |
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