ArtNr |
RF002-5 |
Hersteller |
Agrenvec
|
Menge |
5ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Transforming growth factor beta-3, TGF-beta-3 |
Lieferbar |
|
Molecular Weight: |
Recombinant human TGF-beta3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulphide bond. |
Molecular Formula: |
C600H902N166O174S10 |
p.I: |
6.75 |
Ext. Coeff. Abs (280nm) 0.1% (=1g/l) = |
1.72 |
UniProtKB: |
P10600 |
Endotoxin level: |
Endotoxin Level : < 0.04 EU / ug protein (LAL method) |
Source: |
Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal–derived components or impurities. Animal Free product. |
Description: |
Recombinant human TGF-beta3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulphide bond. Transforming growth factor–beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2 and TGF-beta3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodeling and wound healing. |
Sequence: |
HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLG WKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGL YNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVE QLSNMVVKSCKCS |
Formulation: |
Recombinant human TGF beta-3 is lyophilized from a Tris HCl 0.05M buffer at pH 7.4. |
Purity |
Purity >97% by SDS-PAGE gel |
Applications: |
SDS-PAGE, Western Blot, Antibody Production. |
Bioassay 1 - Result assay 1: |
- |
Bioassay 2 - Result assay 2: |
- |
Bioassay 3 - Result assay 3: |
- |
Bioassay 4 - Result assay 4: |
- |
References: |
-Ten Dijke, P., et al. (1988). Identification of a new member of the transforming growth factor type beta gene family. Proc. Natl. Acad. Sci. USA, 85: 4715-4719. -Massage, J. (1990). The transforming growth factor-beta family. Ann. Rev. Cell Biol., 6: 597-641. -Miller, D.A., et al. (1990). Transforming growth factor beta: a family of growth regulatory peptides. Ann. N.Y. Acad. Sci., 593: 208-217. -Bocharov. E.C., et al. (2002). Dynamics-modulated biological activity of transforming growth factor beta3 J. Biol. Chem., 277(48): 46273-46279. |
RESCONSTITUTION RECOMENDATION QC SHEET |
Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 25-50 ng/ul. Due to the protein nature, dimmers and multimers may be observed. At higher concentration the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines. |
Storage and Stability: |
This lyophilized preparation is stable at 2-8C C for short term, long storage it should be kept at -20C. Reconstituted protein should be stored in working aliquots at –20C. Repeated freezing and thawing is not recommended. |
Shipping |
Room temperature |
Available sizes (ug) : |
1 ug, 5 ug, 10 ug, 500 ug, 100 ug |
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