R2C cells

Disease:

Leydig cell tumor

Origin:

Derived from a 2-month-old male WFu rat.

Species:

Rattus norvegicus, rat

Tissue:

Testis

Morphology:

Epithelial

Properties:

Adherent

Characteristics:

Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Patient:

Male, Rat, 2-month old

Medium:

Hams F12 Medium + 15% horse serum + 2.5% FBS

Subculture:

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10, 000 cells/cm² using 0.25% trypsin/EDTA; Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO