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Sodium/calcium exchanger Background :The sodium-calcium exchanger (NCX) is an antiporter membrane protein that removes calcium from cells. It uses the energy that is stored in the electrochemical gradient of sodium (Na+) by allowing Na+ to flow down its gradient across the plasma membrane in exchange for the countertransport of calcium ions (Ca2+). The NCX removes a single calcium ion in exchange for the import of three sodium ions. The exchanger exists in many different cell types and animal species. The NCX is considered one of the most important cellular mechanisms for removing Ca2+. Expression System : CHO-K1 cells Growth Condition :Ham's F12 + 10% FBS + supplements (details enclosed in shipping Information Sheet) Subculture :1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Morphology and Properties :Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
The sodium-calcium exchanger (NCX) is an antiporter membrane protein that removes calcium from cells. It uses the energy that is stored in the electrochemical gradient of sodium (Na+) by allowing Na+ to flow down its gradient across the plasma membrane in exchange for the countertransport of calcium ions (Ca2+). The NCX removes a single calcium ion in exchange for the import of three sodium ions. The exchanger exists in many different cell types and animal species. The NCX is considered one of the most important cellular mechanisms for removing Ca2+. Expression System : CHO-K1 cells Growth Condition :Ham's F12 + 10% FBS + supplements (details enclosed in shipping Information Sheet) Subculture :1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Morphology and Properties :Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
CHO-K1 cells Growth Condition :Ham's F12 + 10% FBS + supplements (details enclosed in shipping Information Sheet) Subculture :1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Morphology and Properties :Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Ham's F12 + 10% FBS + supplements (details enclosed in shipping Information Sheet) Subculture :1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Morphology and Properties :Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Morphology and Properties :Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Adherent epithelium Gene Name :NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
NCX1, SLC8A1 Sequence :GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
GenBank accession number NM_001112802 Packaging :Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Cryopreserved cells, 1 x 106 cells/vial Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Complete culture medium supplemented with 5% (v/v) DMSO Storage Recommendation :Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Vapor phase of liquid nitrogen Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Hepatitis B: Negative Hepatitis C: Negative Related Products by Target:
Hepatitis C: Negative Related Products by Target:
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