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Homo sapiens, human Tissue: Gall bladder Morphology: Fibroblast Properties: Adherent Cytogenic data: Not specified Patient: Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Gall bladder Morphology: Fibroblast Properties: Adherent Cytogenic data: Not specified Patient: Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Fibroblast Properties: Adherent Cytogenic data: Not specified Patient: Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Adherent Cytogenic data: Not specified Patient: Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Not specified Patient: Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Male, 14 weeks gestation Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Split sub-confluent cultures (70-80%) at a seed ratio of 1:2 is recommended, using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
If use of this culture results in a scientific publication, it should be cited in the publication as: Hs 181.Gb (AddexBio P0037001)
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