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Abelson murine leukemia virus-induced tumor
Established from an ascites of a tumor induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumor targets. Growth inhibited by LPS. Species: Mus musculus Tissue: Blood Morphology: Macrophage/Monocyte Properties: Partial Adherent Patient: Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mus musculus Tissue: Blood Morphology: Macrophage/Monocyte Properties: Partial Adherent Patient: Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Blood Morphology: Macrophage/Monocyte Properties: Partial Adherent Patient: Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Macrophage/Monocyte Properties: Partial Adherent Patient: Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Partial Adherent Patient: Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mouse Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
II Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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