JEG-3 cells

Disease:

Choriocarcinoma

Origin:

This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates by implanting into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. JEG-3 has the same DNA profile as BeWo (C0030001); it was established by serial cloning of BeWo. The cell line forms malignant tumor consistent with choriocarcinoma in nude mice. This cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. The cells are able to transform steroid precursors to estrone and estradiol. This cell line is a suitable transfection host.

Species:

Homo sapiens, human

Tissue:

Placenta

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

modal no. 71

Medium:

AddexBio-formulated EMEM (C0005-01), or equivalent medium + 10% FBS

Subculture:

When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:4 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 7.5% (v/v) DMSO