WERI-Rb-1 cells

Disease:

Retinoblastoma

Origin:

This cell line was isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation in 1974.

Species:

Homo sapiens

Tissue:

Eye, retina

Morphology:

Grape-like clusters of round cells

Properties:

Suspension

Cytogenic data:

This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations

Patient:

Female, 1 yrs of age, Caucasian

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO