AGS cells

Disease:

Gastric adenocarinoma

Origin:

This cell line was derived from fragments of the adenocarcinoma of the stomach from a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Species:

Homo sapiens, human

Tissue:

Stomach

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Hyperdiploid. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1; 8) (q12; p23), der(19)t(19; ?) (q13.6; ?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Patient:

Female, Caucasian, 54 years of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO