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Gastric carcinoma Origin: Derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac Species: Homo sapiens Tissue: Stomach Morphology: Sperical Properties: Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac Species: Homo sapiens Tissue: Stomach Morphology: Sperical Properties: Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Homo sapiens Tissue: Stomach Morphology: Sperical Properties: Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Stomach Morphology: Sperical Properties: Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Sperical Properties: Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Mixed Suspension and Adherent for long term culture Cytogenic data: Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Tumorigenic; The stemline chromosome number is hypotetraploid Patient: Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Male, Asian, 55 yrs of age Medium: IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
IMDM + 20% FBS Subculture: 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium: Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Hepatitis B: Negative Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Hepatitis C: Negative Protein Expression: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; PGM1, 1; PGM3, 1; DNA Profile (STR): Amelogenin: X; CSF1PO: 7, 11; D13S317: 8, 12; D16S539: 10, 12; D5S818: 10, 11; D7S820: 8, 12; THO1: 7, 9; TPOX: 11; vWA: 14, 16 Mutations in KATO III Cells
Mutations in KATO III Cells
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