DU 145

Disease:

Carcinoma

Origin:

The line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. The cells do not express prostate antigen. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments, desmosomes, any mitochondria, well developed Golgi and heterogenous lysosomes.

Species:

Homo sapiens, human

Tissue:

Prostate; derived from metastatic site: brain

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

This is a hypotriploid human cell line. Both 61 and 62 chromosome numbers had the highest rate of occurrence in 30 metaphase counts.The rate of higher ploidies was 3%. The t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32) and 6 other marker chromosomes were found in most cells. The N13 was usually absent. The Y chromosome is abnormal through translocation to an unidentified chromosomal segment. The X chromosome was present in single copy.

Patient:

Caucasian, male, 69 years of age.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:4 to 1:6 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete growth culture medium 95%; DMSO, 5% (v/v) DMSO