H358 cells

Disease:

Bronchioalveolar carcinoma; non-small cell lung cancer

Origin:

This line was derived in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy.

Species:

Homo sapiens, human

Tissue:

Lung/bronchiole; derived from metastatic site: alveolus

Morphology:

Epithelial

Properties:

Adherent

Patient:

Male

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37C. A subcultivation ratio of 1:3 to 1:6 is recommended. Renew medium once every 2 to 3 days depending on cell density.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO