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Adenocarcinoma
This cell line was derived from metastatic site: pleural effusion
Homo sapiens Tissue:Lung, Pleural effusion Morphology:Epithelial Properties: Adherent Cytogenic data:Not available Patient: 50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Lung, Pleural effusion Morphology:Epithelial Properties: Adherent Cytogenic data:Not available Patient: 50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Epithelial Properties: Adherent Cytogenic data:Not available Patient: 50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Adherent Cytogenic data:Not available Patient: 50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Not available Patient: 50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
50+ year-old Caucasian male Medium: RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
RPMI-1640 Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 6 x 10^3 cells/cm2. Maintain culture at 3 x 10^4 cells/cm2. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Hepatitis C: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
Amelogenin: X CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
CSF1PO: 10 D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
D13S317: 11, 12 D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
D16S539: 11, 12 D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
D5S818: 12 D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
D7S820: 9, 12 THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
THO1: 7 TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
TPOX: 8, 9 vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
vWA: 16, 17 If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
If use of this culture results in a scientific publication, it should be cited in the publication as: HCC4006 (AddexBio C0016012) Related cells by Organ: A427 cells A549 cells Calu3 cells H1650 cells H1703 cells H1975 cells H226 cells H23 cells H460 cells H520 cells HCC4006 cells SW1573 cells 3D4/2 cells BEAS-2B cells MRC-5 cells Mv 1 Lu cells RLE-6TN cells
A427 cells
A549 cells
Calu3 cells
H1650 cells
H1703 cells
H1975 cells
H226 cells
H23 cells
H460 cells
H520 cells
HCC4006 cells
SW1573 cells
3D4/2 cells
BEAS-2B cells
MRC-5 cells
Mv 1 Lu cells
RLE-6TN cells
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