Citations |
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Description |
Disease: Colorectal carcinoma Origin: Established from the primary colon carcinoma Species:Homo sapiens Tissue:Colon Morphology:Epithelial Properties:Adherent Cytogenic data:Tumorigenic; the stemline chromosome number is near diploid Patient:Male, adult Medium:AddexBio-Formulated DMEM (C0003-01) + 1X MEM Non-Essential Amino Acids (NEAA)(Thermo 11140050) + 10% FBS or McCoy's 5a Medium + 10% FBS Subculture:1:3 to 1:8 using using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Protein Expression: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin - Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1; PGM1, 1; PGM3, 1 DNA Profile (STR): Amelogenin: X, Y; CSF1PO: 7, 10; D13S317: 10, 12; D16S539: 11, 13; D5S818: 10, 11; D7S820: 11, 12; THO1: 8, 9; TPOX: 8, 9; vWA: 17, 22 Mutations in HCT116 Cells Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-116 (AddexBio C0009005) Related cells by Organ: Caco-2 cells CMT-93 cells COLO 205 cells DLD-1 cells HCA-7 cells HCT-116 cells HCT-8 cells HCT-15 cells HT-29 cells LoVo cells LS-180 cells LS-174T cells RKO cells SNU-407 cells SNU-503 cells SW1116 cells SW48 cells SW480 cells SW620 cells T84 cells |