Vergleich

HCT-116 cells

ArtNr C0009005
Hersteller Addexbio
Menge One Frozen vial
Kategorie
Typ Tumour Cells
Specific against Human (Homo sapiens)
Dry ice Yes
Citations 1.Borralho, P. M., Kren, B. T., Castro, R. E., da Silva, I. B., Steer, C. J., and Rodrigues, C. M. MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells. Febs J, 276: 6689-6700, 2009.
2.Lee, S. J., Cho, Y. H., Kim, H., Park, K., Park, S. K., Ha, S. D., Kim, W. J., and Moon, S. K. Inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer HCT116 cells in vitro and in vivo. Oncol Rep, 22: 1505-1512, 2009.
3.Shin, S., Lee, E. M., Cha, H. J., Bae, S., Jung, J. H., Lee, S. M., Yoon, Y., Lee, H., Kim, S., Kim, H., Lee, S. J., Park, I. C., Jin, Y. W., and An, S. MicroRNAs that respond to histone deacetylase inhibitor SAHA and p53 in HCT116 human colon carcinoma cells. Int J Oncol, 35: 1343-1352, 2009.
4.Yan, T., Seo, Y., and Kinsella, T. J. Differential cellular responses to prolonged LDR-IR in MLH1-proficient and MLH1-deficient colorectal cancer HCT116 cells. Clin Cancer Res, 15: 6912-6920, 2009.
5.Ye, Q., Feng, B., Peng, Y. F., Chen, X. H., Cai, Q., Yu, B. Q., Li, L. H., Qiu, M. Y., Liu, B. Y., and Zheng, M. H. Expression of gamma-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116. World J Gastroenterol, 15: 5035-5043, 2009.
6.Zhu, H., Miao, Z. H., Huang, M., Feng, J. M., Zhang, Z. X., Lu, J. J., Cai, Y. J., Tong, L. J., Xu, Y. F., Qian, X. H., and Ding, J. Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells. Neoplasia, 11: 1226-1234, 2009.
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Lieferbar
Description

Disease:

Colorectal carcinoma

Origin:

Established from the primary colon carcinoma

Species:

Homo sapiens

Tissue:

Colon

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Tumorigenic; the stemline chromosome number is near diploid

Patient:

Male, adult

Medium:

AddexBio-Formulated DMEM (C0003-01) + 1X MEM Non-Essential Amino Acids (NEAA)(Thermo 11140050) + 10% FBS or McCoy's 5a Medium + 10% FBS

Subculture:

1:3 to 1:8 using using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

Protein Expression:

cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -

Isoenzymes:

AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 1; PGM1, 1; PGM3, 1

DNA Profile (STR):

Amelogenin: X, Y; CSF1PO: 7, 10; D13S317: 10, 12; D16S539: 11, 13; D5S818: 10, 11; D7S820: 11, 12; THO1: 8, 9; TPOX: 8, 9; vWA: 17, 22

Mutations in HCT116 Cells

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-116 (AddexBio C0009005)

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Storage condition
LN2
Manufacturers Category
Cell Lines

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: One Frozen vial
Lieferbar: In stock
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