SiHa cells

Disease:

Cervical squamous cell carcinoma

Origin:

This line was established from primary tissue sample obtained from an Asian female patient after surgery. The line is reported to contain an integrated human papillomavirus type 16 genome (HPV-16).

Species:

Homo sapiens

Tissue:

Cervix

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

A hypertriploid human cell line with the modal chromosome number of 71

Patient:

Female, Asian, 55 yrs of age

Medium:

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture:

1:2 to 1:8 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

II

Sterility: