MDA-MB-175VII cells

Disease:

Mammary ductal carcinoma

Origin:

Isolated from pleural effusions.

Species:

Homo sapiens

Tissue:

Mammary gland; breast/duct

Morphology:

Ovoid

Properties:

Epithelial, loosely adherent

Cytogenic data:

model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range.

Patient:

Female, 56 yrs of age, Black

Medium:

AddexBio-formulated Leibovitz's L-15 Medium (C0006-01) + 10% FBS

Subculture:

1:3 to 1:5 using 0.25% trypsin or trypsin/EDTA, without CO2 (or use a non-vented flask and keep the lid closed); 37C

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO