SK-BR-3 cells

Disease:

Breast adenocarcinoma

Origin:

Pleural effusion

Species:

Homo sapiens

Tissue:

Breast; mammary gland

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

This is a hypertriploid to hypotetraploid human cell line(+A, +B, +C, +E, +F, +G, -D) with abnormalities including dicentrics, acrocentric fragments, rings, secondary constrictions, large metacentrics or polycentrics and large submetacentric marker

Patient:

Female, Caucasian, 43 yrs of age

Medium:

McCoy's 5a (Gibco 16600082) + 10% FBS

Subculture:

1:2 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level: