JIMT-1 cells

Disease:

Breast carcinoma

Origin:

Established from pleural effusions of a breast cancer patient (grade 3 invasive, T2N1M0)

Species:

Homo sapiens

Tissue:

Breast, mammary gland

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Human flat-moded highly rearranged hyperdiploid karyotype with 6% polyploidy - 46-56< 2n> XX, +1, +2, +2, +3, +5, +6, +7, +12, -13, -14, -15, -15, -16, -18, +17, +5mar, add(X)(p22.1), add(1)(q11), del(1)(q32.2), der(1)add(1)(p21)add(1)(q22), der(1)t(1; 6)(p11; q1?1)t(6; 5)(q25; ?p13), add(2)(q11)x2, t(2; 3)(q10; p10)add(2)(q25-31), add(3)(p11), der(4)t(?3; 4)(?q24; p11), add(5)(q15), der(5)add(5)(p15.3)del(5)(q22), der(5)t(5; 8)(p11; p11), add(6)(p11)add(6)(q23), der(7)t(1; 7)(p13; q21), der(7)t(7; ?12)(p11; p11), add(8)(p11), i(8q) add(9)(p11), der(9)add(9)(p11)add(9)(q23), add(11)(p13), der(11)dup(11)(p11p15)add(11)(p15), der(11)t(1; 11)(q24; p12), add(12)(q21), der(12; ?15)(p11; q11), add(17)(p11), add(17)(p13), der(17)add(17)(p11)add(17)(q21)

Patient:

Female, 62 yrs of age

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

1:2 to 1:6 (80-90%); using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility: