Disease:
Glioblastoma
Origin:
The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma.
Species:
Homo sapiens, human Tissue:
Brain/right frontal parieto-occipital cortex Morphology:
Epithelial Properties:
Adherent Patient:
Caucasian, female, 60 years of age Medium:
AddexBio-formulated DMEM (C0003-01) + 5% FBS Subculture:
Subculture at a ratio of 1:4 to 1:6 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:
Complete growth culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative DNA Profile (STR):
Amelogenin: X CSF1PO: 12 D13S317: 10, 11 D16S539: 12 D5S818: 11, 12 D7S820: 8, 11 THO1:9.3 TPOX: 8 vWA: 16, 19 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: LN-229 (AddexBio C0005017)