Disease:
Glioblastoma (Multiform)
Origin:
Undifferentiated glioblastoma Species:
Homo sapiens Tissue:
CNS Properties:
Adherent Cytogenic data:
5n+/-, Near-pentaploid 115+/- (104-126) Patient:
Female, 67 yrs of age Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:
1:5 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 C Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative DNA Profile (STR):
Amelogenin: X, X CSF1PO: 10, 13 D13S317: 10, 10 D16S539: 12, 13 D5S818: 11, 12 D7S820: 9, 13 THO1: 9.3, 10 TPOX: 8, 11 vWA: 16, 17 Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SF-295 (AddexBio C0005005) Mutations in SF-295 Cells