CCRF-CEM cells

Lymphoblast

Species:

Homo sapiens

Tissue:

Blood

Morphology:

Lymphoblast

Properties:

Suspension

Cytogenic data:

CCRF-CEM cells were derived from the peripheral blood buffy coat of a child (CEM) with acute lymphoblastic leukemia who had originally presented with lymphosarcoma

Patient:

Female, Caucasian, 4 yrs of age

Medium:

RPMI1640 + 10% FBS

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10⁵ viable cells/ml. Maintain cell density between 1 to 2 X 10⁶ viable cells/ml.

Medium Renewal:

Add fresh medium (20% to 30% by volume) every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level: