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Recombinant Mouse SCF Protein Europäischer Partner

Hersteller Abclonal
Kategorie
Typ Proteins Recombinant
Specific against Mouse
Menge 100 ug
Host Mouse
ArtNr RP01055-100ug
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Alias KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL
Purity > 95% by SDS-PAGE.
Description
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Route
C-His
Manufacturers Category
Proteins
Endotoxin
< 0.1 EU/μg of the protein by LAL method.
Immunogen
Lys26-Ala189
Storage
Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Manufacturers Research Area
Cytokines & Cytokine receptors, Cell Culture related
Gene Symbol
c-Kit ligand/SCF/KITLG
Protein Formulation
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Protein Description
Recombinant Mouse c-Kit ligand/SCF/KITLG Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys26-Ala189) of mouse SCF (Accession #NP_038626.1.) fused with a 6×His tag at the C-terminus.
Protein Bio Activity
1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×106-2.0×106 units/mg.|2. Measured by its binding ability in a functional ELISA.Immobilized recombinant Mouse SCF at 2 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1.5-10 ng/mL.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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