Hersteller |
Abclonal
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Mouse |
Menge |
100 ug |
Host |
Mouse |
ArtNr |
RP01055-100ug |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Alias |
KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL |
Purity |
> 95% by SDS-PAGE. |
Description |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Route |
C-His |
Manufacturers Category |
Proteins |
Endotoxin |
< 0.1 EU/μg of the protein by LAL method. |
Immunogen |
Lys26-Ala189 |
Storage |
Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. |
Manufacturers Research Area |
Cytokines & Cytokine receptors, Cell Culture related |
Gene Symbol |
c-Kit ligand/SCF/KITLG |
Protein Formulation |
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation. |
Protein Description |
Recombinant Mouse c-Kit ligand/SCF/KITLG Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Lys26-Ala189) of mouse SCF (Accession #NP_038626.1.) fused with a 6×His tag at the C-terminus. |
Protein Bio Activity |
1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×106-2.0×106 units/mg.|2. Measured by its binding ability in a functional ELISA.Immobilized recombinant Mouse SCF at 2 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1.5-10 ng/mL. |
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