Annexin V-AbFluor 555 Apoptosis Detection Kit

Abbkine Annexin V-AbFluor 555 Apoptosis Detection Kit contains Annexin V labeled with our proprietary orange fluorescent dye AbFluor 555, which allows the identification and quantification of apoptotic cells by flow cytometry or fluorescence microscopy.

Abbkine AbFluor 555 dye provides the same or better fluorescence and photostability as Alexa Fluor 555, Cy3, TRITC

Annexin V- AbFluor 555: Abs/Em = 555/565 nm

Suitable for flow cytometry or fluorescence microscopy

Blue ice

Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet.