Rabbit Interleukin 27 (IL-27) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rabbit Interleukin 27 microplate
Rabbit Interleukin 27 standard
Rabbit Interleukin 27 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Interleukin 27 is a non-covalently linked heterodimeric cytokine that is structurally related to IL-12. It is composed of an Epstein-Barr virus-induced molecule 3 subunit linked with the IL-27 p28 subunit. IL-27 is produced by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells.Interleukin-30 (IL-30) is a protein with a molecular weight of 28 kilodaltons, which forms one chain of the heterodimeric cytokine called interleukin 27 (IL-27), thus is sometimes called IL27-p28. The other chain of IL-27 is a molecule called Epstein-Barr induced gene-3 (EBI3). IL-30 is a member of the long-chain, 4-helix bundle family of cytokines, making it structurally similar to IL-6. This gene for this molecule is now officially called IL-27 under HGNC guidelines.