Rabbit Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rabbit Tumor necrosis factor-inducible gene 6 protein microplate
Rabbit Tumor necrosis factor-inducible gene 6 protein standard
Rabbit Tumor necrosis factor-inducible gene 6 protein detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IalphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation. The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1). The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.

TNFAIP6; TSG-6; TSG6; hyaluronate-binding protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein