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Rat Stathmin (STMN1) ELISA Kit

ArtNr ABK-KTE100137-48T
Hersteller Abbkine Scientific
Menge 48T
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Rat Stathmin (STMN1) ELISA Kit employs a two-site sandwich ELISA to quantitate STMN1 in samples. An antibody specific for STMN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySTMN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STMN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STMN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Rat Stathmin microplate
Rat Stathmin standard
Rat Stathmin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Rat Stathmin (STMN1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat STMN1. No significant cross-reactivity or interference between Rat STMN1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Stathmin 1/oncoprotein 18, also known as STMN1, is a highly conserved 17 kDa protein. Its function as an important regulatory protein of microtubule dynamics has been well characterized. Eukaryotic microtubules are one of three major components of the cell’s cytoskeleton. They are highly dynamic structures that continuously alternate between assembly and disassembly. Stathmin performs an important function in regulating rapid microtubule remodeling of the cytoskeleton in response to the cell’s needs. Microtubules are cylindrical polymers of alpha, beta-tubulin. Their assembly is in part determined by the concentration of free tubulin in the cytoplasm.At low concentrations of free tubulin, the growth rate at the microtubule ends is slowed and results in an increased rate of depolymerization (disassembly).
Gene ID
29332
Alternative
STMN1; RP1-125I3.5; C1orf215; FLJ32206; LAP18; Lag; MGC138869; MGC138870; OP18; PP17; PP19; PR22; SMN; OTTHUMP00000008530; leukemia-associated phosphoprotein p18; metablastin; oncoprotein 18; phosphoprotein 19
Accession
P13668

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48T
Lieferbar: In stock
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