Vergleich

Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit

ArtNr ABK-KTE100073-96Tx5
Hersteller Abbkine Scientific
Menge 96Tx5
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP8L2 in samples. An antibody specific for TNFAIP8L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP8L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP8L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP8L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 microplate
Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 standard
Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Rat Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFAIP8L2. No significant cross-reactivity or interference between Rat TNFAIP8L2 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Sun et al. (2008) cloned mouse Tnfaip8l2, which they called Tipe2, and by database analysis, they identified human TNFAIP8L2. The deduced 184-amino acid human protein shares 94% identity with mouse Tnfaip8l2, shares 53% identity with human TNFAIP8 , and contains a death effector domain (DED) and 6 putative alpha-helices characteristic of DED-containing proteins. Northern blot analysis detected strong expression in mouse thymus, spleen, lymph node, and small intestine, with weak expression in lung, skin, and colon. Tnfaip8l2 was also expressed in murine lymphoid cells and cell lines, in inflamed mouse spinal cord, and in TNF-alpha-stimulated mouse fibroblasts, suggesting that Tnfaip8l2 is preferentially expressed in lymphoid tissue.
Gene ID
100328663
Alternative
TNFAIP8L2; RP11-68I18.4; FLJ23467; TIPE2; inflammation factor 20
Accession
B7NZC7

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen