Hersteller |
Abbkine Scientific
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Porcine |
Applikationen |
ELISA |
Menge |
96Tx5 |
ArtNr |
ABK-KTE80103-96Tx5 |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Lieferbar |
|
Applications notes |
This Pig Melatonin receptor type 1A (MTNR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate MTNR1A in samples. An antibody specific for MTNR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTNR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTNR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTNR1A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Pig Melatonin receptor type 1A microplate Pig Melatonin receptor type 1A standard Pig Melatonin receptor type 1A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Pig Melatonin receptor type 1A (MTNR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Pig MTNR1A. No significant cross-reactivity or interference between Pig MTNR1A and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
MTNR1A encodes the MT1 protein, one of two high-affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G protein-coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. |
Alternative |
MTNR1A; MEL-1A-R; MT1; melatonin receptor type 1A |
Accession |
O02781 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.