Mouse Galectin-1 (LGALS1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Galectin-1 microplate
Mouse Galectin-1 standard
Mouse Galectin-1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Galectin-1 is one of the most important lectins to date participating in the malignant tumor development. With more and more data available on its expression in tumors it is already clear that this lectin is an important target for cancer diagnostics and treatment.Its first involvement in cancer was associated with cell adhesion. Later on the lectin was shown to induce apoptosis of activated T-cells, and experiments have confirmed that cancer cells express galectin-1 also to protect themselves from immune response. Latest finding is a participation of galectin-1 in tumor angiogenesis as an angiogenic factor. In most of the cases described, galectin-1 is up-regulated in tumors in comparison to normal tissue. Moreover, the presence of the lectin is associated with metastases and poor prognosis of disease outcome.

LGALS1; DKFZp686E23103; GAL1; GBP; S-Lac lectin 1; beta-galactoside-binding lectin L-14-I; galaptin; galectin 1; galectin-1; lactose-binding lectin 1