ArtNr |
ABK-KTE70482-96T |
Hersteller |
Abbkine Scientific
|
Menge |
96T |
Kategorie |
|
Typ |
Elisa-Kit |
Applikationen |
ELISA |
Specific against |
Mouse |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Lieferbar |
|
Applications notes |
This Mouse Sphingosine 1-phosphate receptor 4 (S1PR4) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR4 in samples. An antibody specific for S1PR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Mouse Sphingosine 1-phosphate receptor 4 microplate Mouse Sphingosine 1-phosphate receptor 4 standard Mouse Sphingosine 1-phosphate receptor 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Mouse Sphingosine 1-phosphate receptor 4 (S1PR4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S1PR4. No significant cross-reactivity or interference between Mouse S1PR4 and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
S1PR4 is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). Hence this receptor is also known as S1P4.This gene is a member of the endothelial differentiation, G-protein-coupled (EDG) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue.Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung. |
Gene ID |
13611 |
Alternative |
S1PR4; EDG6; LPC1; S1P4; SLP4; Sphingosine 1-phosphate receptor 4; Sphingosine 1-phosphate receptor Edg-6; endothelial differentiation; G protein coupled receptor 6; endothelial differentiation; G-protein-cou |
Accession |
Q9Z0L1 |
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