Vergleich

Mouse Sphingosine 1-phosphate receptor 4 (S1PR4) ELISA Kit

ArtNr ABK-KTE70482-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Sphingosine 1-phosphate receptor 4 (S1PR4) ELISA Kit employs a two-site sandwich ELISA to quantitate S1PR4 in samples. An antibody specific for S1PR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS1PR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S1PR4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S1PR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Sphingosine 1-phosphate receptor 4 microplate
Mouse Sphingosine 1-phosphate receptor 4 standard
Mouse Sphingosine 1-phosphate receptor 4 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Sphingosine 1-phosphate receptor 4 (S1PR4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse S1PR4. No significant cross-reactivity or interference between Mouse S1PR4 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
S1PR4 is a G protein-coupled receptor which binds the lipid signaling molecule sphingosine 1-phosphate (S1P). Hence this receptor is also known as S1P4.This gene is a member of the endothelial differentiation, G-protein-coupled (EDG) receptor gene family. EDG receptors bind lysophospholipids or lysosphingolipids as ligands, and are involved in cell signalling in many different cell types. This EDG receptor gene is intronless and is specifically expressed in the lymphoid tissue.Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung.
Gene ID
13611
Alternative
S1PR4; EDG6; LPC1; S1P4; SLP4; Sphingosine 1-phosphate receptor 4; Sphingosine 1-phosphate receptor Edg-6; endothelial differentiation; G protein coupled receptor 6; endothelial differentiation; G-protein-cou
Accession
Q9Z0L1

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen