Mouse Secretin (SCT) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Mouse Secretin microplate
Mouse Secretin standard
Mouse Secretin detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body. Acting as an anti-Müllerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkühn. Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate. It is notable for being the first hormone to be identified. In humans, the secretin peptide is encoded by the SCT gene. It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney.
Secretin is a linear peptide hormone, which is composed of 27 amino acids and has a molecular weight of 3055. A helix is formed in the amino acids between positions 5 and 13.

SCT