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Mouse Mitochondrial import receptor subunit TOM40B (TOMM40L) ELISA Kit

ArtNr ABK-KTE70072-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Mitochondrial import receptor subunit TOM40B (TOMM40L) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM40L in samples. An antibody specific for TOMM40L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM40L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM40L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM40L bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Mitochondrial import receptor subunit TOM40B microplate
Mouse Mitochondrial import receptor subunit TOM40B standard
Mouse Mitochondrial import receptor subunit TOM40B detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Mitochondrial import receptor subunit TOM40B (TOMM40L) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TOMM40L. No significant cross-reactivity or interference between Mouse TOMM40L and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
TOMM40L belongs to the Tom40 family. Potential channel-forming protein implicated in import of protein precursors into mitochondria. TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria.Extraction and antibody-accessibility studies suggested that Tomm40 is embedded within the mitochondrial membrane.Tomm40 was associated with a 400-kD complex containing the mitochondrial import receptors Tom20 and Tom22 and several smaller components. Mitochondria-targeted preproteins interacted with the Tomm40-containing complex during their transit to the mitochondrial matrix, and immunodepletion of Tomm40 caused the loss of preprotein import activity.
Gene ID
641376
Alternative
TOMM40L; FLJ12770; RP11-297K8.10; TOMM40B; OTTHUMP00000032243; translocase of outer mitochondrial membrane 40 homolog-like
Accession
Q9CZR3

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
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