Hersteller |
Abbkine Scientific
|
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human |
Applikationen |
ELISA |
Menge |
96T |
ArtNr |
ABK-KTE62724-96T |
eClass 6.1 |
32160605 |
eClass 9.0 |
32160605 |
Lieferbar |
|
Applications notes |
This Human Zika Virus IgG antibody (ZV-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate ZV-Ab-IgG in samples. An antibody specific for ZV-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZV-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZV-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZV-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human Zika Virus IgG antibody microplate Human Zika Virus IgG antibody standard Human Zika Virus IgG antibody detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human Zika Virus IgG antibody (ZV-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human ZV-Ab-IgG. No significant cross-reactivity or interference between Human ZV-Ab-IgG and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
Zika virus (ZIKV) is a member of the virus family Flaviviridae.[3] It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus. Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947. Zika virus is related to the dengue, yellow fever, Japanese encephalitis, and West Nile viruses. Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. From 2007 to 2016, the virus spread eastward, across the Pacific Ocean to the Americas, leading to the 2015–16 Zika virus epidemic. |
Alternative |
ZV-Ab-IgG |
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