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Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96T
ArtNr ABK-KTE62700-96T
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyd-Ab-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Hyd-Ab-IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled Hyd-Ab-IgM and unlabeled Hyd-Ab-IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Hyd-Ab-IgM in the sample. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Hydatid IgM antibody microplate
Human Hydatid IgM antibody standard
Human Hydatid IgM antibody detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Hydatid IgM antibody (Hyd-Ab-IgM) ELISA Kit has high sensitivity and excellent specificity for detection of Human Hyd-Ab-IgM. No significant cross-reactivity or interference between Human Hyd-Ab-IgM and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Hydatid disease in man is caused mainly by infection by the larval stage of the dog tapeworm Echinococcus granulosus. It follows accidental ingestion of tapeworm eggs excreted in the faeces of infected dogs. Worldwide, four species of tapeworm are clinically important to man: E. granulosus, Echinococcus multilocularis, Echinococcus vogeli and Echinococcus oligarthrus, but only E. granulosus has been found in the UK. Hydatid disease is one of the most geographically widespread zoonoses. The natural hosts are canine predators, particularly domestic dogs and foxes (mainly the Arctic fox and the red fox). The condition is complicated and expensive to treat, and may require extensive surgery and prolonged drug therapy. The World Health Organization (WHO) aims for an effective disease control strategy by 2018.
Alternative
Hyd-Ab-IgM

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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