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Human Homeobox protein CDX-2 (CDX2) ELISA Kit

ArtNr ABK-KTE62284-96Tx5
Hersteller Abbkine Scientific
Menge 96Tx5
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Human Homeobox protein CDX-2 (CDX2) ELISA Kit employs a two-site sandwich ELISA to quantitate CDX2 in samples. An antibody specific for CDX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCDX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CDX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Homeobox protein CDX-2 microplate
Human Homeobox protein CDX-2 standard
Human Homeobox protein CDX-2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Homeobox protein CDX-2 (CDX2) ELISA Kit has high sensitivity and excellent specificity for detection of Human CDX2. No significant cross-reactivity or interference between Human CDX2 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Caudal type homeobox transcription factor 2 is a homeobox transcription factor.Cdx2 is the gene that directs early embryogenesis in mice. It is required to form the placenta.Ectopic expression of CDX2 was reported more than 85% of the human patients with Acute myeloid leukemia (AML). Ectopic expression of Cdx2 in murine bone marrow induced AML in mice and upregulate Hox genes in bone marrow progenitors.This gene (or, more specifically, the equivalent gene in humans) has come up in the proposal by the President's Council on Bioethics, as a solution to the stem cell controversy. According to one of the plans put forth, by deactivating the gene, it would not be possible for a properly organized embryo to form, thus providing stem cells without requiring the destruction of an embryo.
Gene ID
1045
Alternative
CDX2; CDX-3; CDX3; caudal type homeo box transcription factor 2; caudal type homeobox transcription factor 2
Accession
Q99626

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
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