Vergleich

Human Protein artemis (DCLRE1C) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx5
ArtNr ABK-KTE62129-96Tx5
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Protein artemis (DCLRE1C) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLRE1C in samples. An antibody specific for DCLRE1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLRE1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLRE1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLRE1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Protein artemis microplate
Human Protein artemis standard
Human Protein artemis detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Protein artemis (DCLRE1C) ELISA Kit has high sensitivity and excellent specificity for detection of Human DCLRE1C. No significant cross-reactivity or interference between Human DCLRE1C and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
DCLRE1C encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The encoded protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Mutations in DCLRE1C can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants.
Gene ID
64421
Alternative
DCLRE1C; RP11-398C13.5; A-SCID; DCLREC1C; FLJ11360; FLJ36438; RS-SCID; SCIDA; SNM1C; OTTHUMP00000019166; OTTHUMP00000019167; OTTHUMP00000019168; OTTHUMP00000019170; OTTHUMP00000019171; OTTHUMP00000019172; artemis
Accession
Q96SD1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
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