ArtNr |
ABK-KTE62101-96Tx5 |
Hersteller |
Abbkine Scientific
|
Menge |
96Tx5 |
Kategorie |
|
Typ |
Elisa-Kit |
Applikationen |
ELISA |
Specific against |
Human |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Lieferbar |
|
Applications notes |
This Human Sphingolipid delta (DEGS1) ELISA Kit employs a two-site sandwich ELISA to quantitate DEGS1 in samples. An antibody specific for DEGS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDEGS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEGS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEGS1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Detection method |
Colorimetric |
Sample type |
Cell culture supernatants#Serum#Plasma#Other biological fluids |
Assay type |
Sandwich ELISA (quantitative) |
Assay duration |
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
Kit components |
Human Sphingolipid delta microplate Human Sphingolipid delta standard Human Sphingolipid delta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers |
Features & Benefits |
Human Sphingolipid delta (DEGS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DEGS1. No significant cross-reactivity or interference between Human DEGS1 and analogues was observed. |
Usage notes |
Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate. |
Storage instructions |
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols. |
Shipping |
Gel pack with blue ice. |
Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background |
DEGS1 encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of DEGS1 inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. |
Gene ID |
8560 |
Alternative |
DEGS1; DEGS; DES1; Des-1; FADS7; MGC5079; MIG15; MLD; degenerative spermatocyte homolog 1; lipid desaturase; degenerative spermatocyte homolog; lipid desaturase; dihydroceramide desaturase; membrane fatty aci |
Accession |
O15121 |
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