Human Methotrexate (MTX) ELISA Kit

This Human Methotrexate (MTX) ELISA Kit employs a two-site sandwich ELISA to quantitate MTX in samples. An antibody specific for MTX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTX bound in the initial step. The color development is stopped and the intensity of the color is measured.

Cell culture supernatants#Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Human Methotrexate (MTX) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTX. No significant cross-reactivity or interference between Human MTX and analogues was observed.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

MTX